Skin is the barrier between the internal and external environment and is the largest organ of the human body. After a skin injury, the damaged tissue is restored through coordinated signaling that constitutes the cutaneous healing response. Due to deficiency of donor skin graft supplies, tissue-engineered skin substitutes present an efficient alternative in substituting donor skin grafts. Skin substitutes act as a temporary protective cover of the wound bed, thus protecting damaged regions from fluid loss and contamination along with accelerating the wound-healing processes.

Due to the presence of stem cells, the wounded epidermis is able to stimulate self-regeneration. Tissue engineered skin substitutes are upcoming alternatives to traditional wound healing strategies and tissue regeneration. Skin substitutes derived from adipose tissue, bone-marrow, and peripheral blood for wound healing and tissue regeneration.